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no. 13256-029), and 100 U/ml streptomycin and penicillin (cat. no. 15070063; all from Thermo Fisher Scientific, Waltham, MA, USA); and 100 mg/l heparin (cat. no. H3149; Sigma–Aldrich, St. Louis cells Article Characterization of Carotid Smooth Muscle Cells during Phenotypic Transition Haize Goikuria 1,2, Maria del Mar Freijo 3, Reyes Vega Manrique 4, María Sastre 1,2, Elena Elizagaray 5, Ana Lorenzo 3, Koen Vandenbroeck 1,2,6 and Iraide Alloza 1,2,6,* 1 Neurogenomiks Neuroscience Department, Faculty of Medicine and Nursing, Basque Country University, Endothelial cells were grown in “normal growth medium” (NGM): MCDB 131 (Caisson Labs, Carlsbad, CA) supplemented with 10% heat inactivated fetal bovine serum (F0926; Sigma), 25 mg/mL endothelial mitogen (BT-203, Biomedical Technologies), 2 U/mL heparin (H3149, Sigma), 1 μg/mL hydrocortisone (H0888, Sigma), 0.2 mM ascorbic acid 2-phosphate (49752, Sigma), and 1% penicillin-streptomycin HUDEP-2 cells were differentiated in a two-phase differentiation protocol consisting of IMDM supplemented with 5% human AB serum (H6914, Sigma), 10μg/mL recombinant human insulin (91077C, Sigma), 330μg/mL holo-transferrin (T4132, Sigma), 3 units/mL EPO (Amgen), 1μg/mL doxycycline (D9891, Sigma), 2 units/mL heparin (H3149, Sigma), and 1% penicillin/streptomycin.

Prior to use, the stock solution was further diluted by M199 media for obtainment of working solutions with different concentrations of 0.1, 0.3, and 1 mmol/L. HUVECs were pretreated with the working solutions of paeoniflorin at 37 °C with 5% 2021-02-12 2021-02-24 2020-11-01 2020-01-23 Neural stem cells were expanded on Matrigel (CORNING 356230) or fibronectin-coated 6-well plate in stem cell growth medium (GM) containing DEME/F12 plus Glutamax (Life Technologies 10565-018), BIT9500 Serum Substitute (1x, Stem Cell Technologies 09500), Heparin (20ug/mL Sigma H3149), Primocin (1x, InvivoGen ant-pm-1), human bFGF (20ng/Ml, Stemgent 03-0002), human EGF (20ng/mL, … (37°C; Sigma-Aldrich) and transferred to DMEM containing 2mML-glutamine, 100 U/ml penicillin and streptomycin, and 10% FCS (Sigma-Aldrich). Confluent astrocyte cultures were treated with mitomycin C (10 mg/ml; Sigma-Aldrich) for 2 hours to elim-inate proliferating cells, followed by replacement of medium to prewarmed fresh medium for 24 hours. Article Title: Optimizing miR-29 Measurements in Biobanked, Heparinized Samples Article Snippet: ..Three types of heparinases and two individual manufacturers were tested; recombinant F. Heparinum heparinase I (E. coli derived, #7897-GH-010, R & D systems Minneapolis, MN, USA); recombinant P. heparinus heparinase II (E.

50 ng/mL human SCF (300-07, Peprotech) was included in the phase one of the culture (day 1-3) and withdrawn in the phase two of Heparin (H3149, Sigma-Aldrich, St. Louis, MO, USA), 5% newborn calf serum (N4762, Sigma-Aldrich, St. Louis, MO, USA), 0.2 g/mL bovine serum albumin (BSA) (A9418, Sigma-Aldrich, St. Louis, MO, USA), 2 mM L-glutamine (G7513, Sigma-Aldrich, St. Louis, MO, USA), 100 g/mL Streptomycin, and #T4132), 1% penicillin/streptomycin, 10ng/ml heparin (Sigma, H3149), 10ug/ml insulin (Sigma, Cat. #I9278), 1ug/ml doxycycline. Peripheral blood mononuclear cells (PBMCs) were obtained from the University of Pennsylvania Human Immunology Core. 2020-10-13 · High Five™ cells (#B85502, Gibco) were cultured in Express Five SFM (#10486025, Gibco) supplemented with 18 mM L-glutamine (#25030081, Gibco), 10 U/mL heparin (#H3149, Sigma-Aldrich), and 0.25% penicillin-streptomycin.

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primer · Shindaiwa startapparater · Shindaiwa startfjädrar · Shindaiwa tanklock · Shindaiwa vibrationsdämpare · Sigma reservdelar · Sigma gräsklipparknivar. H3149), 100 μM sodium orthovanadate (Sigma-Aldrich, no. S6508), 50 μg ml −1 ascorbic acid (Sigma-Aldrich, no. A4544), 2 mM L-glutamine (Gibco Invitrogen, primer · Shindaiwa startapparater · Shindaiwa startfjädrar · Shindaiwa tanklock · Shindaiwa vibrationsdämpare · Sigma reservdelar · Sigma gräsklipparknivar.

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Add to cart. SKU: H3149-100KU Category: SIGMA. Search Product. Search for: Browse by Supplier. Agilent Technologies; Bio-Rad; Life Technologies (Thermo … Please contact us for the closest replacement -Heparin sodium salt from porcine intestinal mucosa, Grade I-A, >=180 USP units/mg, powder, BioReagent, suitable for cell culture (H3149-50KU) Sigma Aldrich Miscellaneous Products H3149-50KU H3149-250KU Description Heparin sodium salt from porcine intestinal mucosa, Grade I-A, =180 USP units/mg, powder, BioReagent, suitable for cell culture, 250 KU … 2020-10-13 We injected 10 mL of PBS/Heparin (Cat# H3149, Sigma-Aldrich, 10 U/mL) and 10 mL of 4% paraformaldehyde solution consecutively at a rate of 2 mL/min into left ventricle of the mice heart. Gastrocnemius muscles were collected after perfusion fixation. Endothelial cells were grown in “normal growth medium” (NGM): MCDB 131 (Caisson Labs) supplemented with 10% heat-inactivated FBS (F0926; Sigma), 25 mg/mL endothelial mitogen (BT-203, Biomedical Technologies), 2 U/mL heparin (H3149, Sigma), 1 μg/mL hydrocortisone (H0888, Sigma), 0.2 mmol/L ascorbic acid 2-phosphate (49752, Sigma), and 1% penicillin–streptomycin–glutamine … HUDEP-2 cells were differentiated in a two-phase differentiation protocol consisting of IMDM supplemented with 5% human AB serum (H6914, Sigma), 10μg/mL recombinant human insulin (91077C, Sigma), 330μg/mL holo-transferrin (T4132, Sigma), 3 units/mL EPO (Amgen), 1μg/mL doxycycline (D9891, Sigma), 2 units/mL heparin (H3149, Sigma), and 1% penicillin/streptomycin.

SKU: H3149-100KU Category: SIGMA. Search Product. Search for: Browse by Supplier. Agilent Technologies; Bio-Rad; Life Technologies (Thermo … Please contact us for the closest replacement -Heparin sodium salt from porcine intestinal mucosa, Grade I-A, >=180 USP units/mg, powder, BioReagent, suitable for cell culture (H3149-50KU) Sigma Aldrich Miscellaneous Products H3149-50KU H3149-250KU Description Heparin sodium salt from porcine intestinal mucosa, Grade I-A, =180 USP units/mg, powder, BioReagent, suitable for cell culture, 250 KU … 2020-10-13 We injected 10 mL of PBS/Heparin (Cat# H3149, Sigma-Aldrich, 10 U/mL) and 10 mL of 4% paraformaldehyde solution consecutively at a rate of 2 mL/min into left ventricle of the mice heart. Gastrocnemius muscles were collected after perfusion fixation. Endothelial cells were grown in “normal growth medium” (NGM): MCDB 131 (Caisson Labs) supplemented with 10% heat-inactivated FBS (F0926; Sigma), 25 mg/mL endothelial mitogen (BT-203, Biomedical Technologies), 2 U/mL heparin (H3149, Sigma), 1 μg/mL hydrocortisone (H0888, Sigma), 0.2 mmol/L ascorbic acid 2-phosphate (49752, Sigma), and 1% penicillin–streptomycin–glutamine … HUDEP-2 cells were differentiated in a two-phase differentiation protocol consisting of IMDM supplemented with 5% human AB serum (H6914, Sigma), 10μg/mL recombinant human insulin (91077C, Sigma), 330μg/mL holo-transferrin (T4132, Sigma), 3 units/mL EPO (Amgen), 1μg/mL doxycycline (D9891, Sigma), 2 units/mL heparin (H3149, Sigma), and 1% penicillin/streptomycin. 50 ng/mL human SCF … 2021-01-21 2015-04-01 The papain was inactivated using ovomucoid solution (cat# 542000, SciQuest), tissue was briefly treated with DNase (cat# 10104159001, Sigma) and passed through the 100μm cell strainer (cat# 542000, Greiner Bio-One).
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HEPES, Sigma Låt 2 ml blod (1-5 x 10 6 celler) på 1 ml Ficoll Histopaque 1,077 (Sigma) Heparin natriumsalt (Sigma-Aldrich H3149-50KU); Vid odling av Xenopus 15 / 0 src/ui_searchDialogGui.h:891 9754 msgid "sigma" 9755 msgstr "sigma" 9756 10229 10230 #: src/ui_viewDialog.h:3149 10231 msgid "Barnard's Catalogue av J Ekelund · 2019 — Om inget annat anges så köptes samtliga reagenser in från Sigma Aldrich, Stockholm µg/ml heparin (H3149) och PHE med en slutlig koncentration på 1,7 mM PPG, Sigma primer Sigmacover 280, 5 liters 298,03 € PPG, Sigma EcoFleet 530, bottenfärg , svart / blå / röda brun, 20 liter 919,81 F Harken H 3149. 167,90 av A Lundgren · 2014 — arbetskraft = (5510 kr*16 h)/28 h = 3149 kr arbetskraft = (5590 kr*12 h)/28 h = 2396 kr Liknande Sex Sigma metoden. Viktigt att inte fokusera apt J " Fredk T (Lucretia M) slsmn Scott Paper Co h3149 35th av S " Jas r816y W " Mary elk r4606 Juneau " Ray house mgr Delta Sigma Phi Fraternity r4550 tid i kostnadsstället)/total summa av direkt arbetskraft = (5510 kr*16 h)/28 h = 3149 kr Totalbatchkostnad av batch A= 5149 kr Liknande Sex Sigma metoden.
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Feb 26, 2020 FBS, 30 µg/ml heparin (Sigma, H3149), and 10 ng/ml TGF-β1 (Peprotech, Cells were stained with 0.5% crystal violet solution (Sigma,. Human Apo-Transferrin (Sigma-Aldrich; T2036) + Heparin 10µg/ml (Sigma; H3149).

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2 µg/mL heparan sulfate (Catalog No. H3149, Sigma) Subculturing The cells grow as phase-bright, smooth spheres. The neurospheres should not get too big, ragged or dark as this is a sign of unhealthy, dying cells. The cells should be passaged when the neurospheres are about 200-400 μm in size. RAD–Heparin composites were prepared combining 95 μL of RAD16-I 0.5% (w/v) and 5 μL of heparin sodium salt solution (H3149, Sigma) in a concentration range between 0.01% and 1% (w/v), control RAD16-I samples were prepared with a final concentration of 0.5% (w/v). 100 μL of each type of sample were loaded into a cell culture insert (PICM-1250, Millipore) previously placed into a 6-well culture plate, and 500 μL of PBS were added under the insert to star the self-assembling process. HUDEP-2 cells were differentiated in a two-phase differentiation protocol consisting of IMDM supplemented with 5% human AB serum (H6914, Sigma), 10μg/mL recombinant human insulin (91077C, Sigma), 330μg/mL holo-transferrin (T4132, Sigma), 3 units/mL EPO (Amgen), 1μg/mL doxycycline (D9891, Sigma), 2 units/mL heparin (H3149, Sigma), and 1% growth factor (VEGF; V7259, Sigma-Aldrich) and 10 U/mL heparin (H3149, Sigma-Aldrich).